I will like the same writer to handle this project as well. The writer ID 303. The writer should please check the attached file for instructions for the project.

cell Biology

Purification of Bovine Brain Tubulins
Source: Banerjee, A., M. C. Roach, P. Treka, R. F. Luduena. 1990. Increased microtubule assembly in bovine brain tubulin lacking the type III isotype of β-tubulin. J. Biol. Chem. 265: 1794–1799.
Corresponding chapter(s) in the textbook: Chapter 13 (and 4)
Review the following terms before working on the problem: cytoskeleton, microtubules, tubulins, agarose, column chromatography, polyacrylamide gel electrophoresis, Coomassie Blue staining, Western blotting
Read the paper and answer the questions below that refers to the data described in Figure 1 of the paper.
Be prepared to discuss the other experiments described, in class.

Experiment
Tubulin was isolated from bovine brain, purified by phosphocellulose ion exchange chromatography, and further fractionated by affinity chromatography on an anti-2-tubulin-agarose column. (Note: Affinity chromatography is a powerful separation technique based on the specific, noncovalent interaction between two molecules; in this experiment: between β2-tubulin and its antibody. The antibody is immobilized on agarose beads, and the protein solution is passed through the antibody-agarose column. Unbound molecules are washed out with excess sample buffer, and the specifically bound proteins are eluted with a high-salt solution.)
The original tubulin (PC, for phosphocellulose-purified), the unbound fraction (U), and a bound fraction eluted from the column (B) were fractionated by polyacrylamide gel electrophoresis. The gels were subjected to the following procedures:
(a) Coomassie Blue protein staining
(b) Western blot with anti-2-tubulin
(c) Western blot with anti-1-tubulin
Figure

Questions
1. Assess the molar ratio of α- and β-tubulins in the phosphocellulose-purified sample (sample 1). Explain your answer.
2. What conclusion can be drawn from comparing samples 1 and 2?
3. What conclusion can be drawn from comparing samples 1 and 3?
4. Evaluate the specificity of the anti-2-tubulin antibody used in the experiment.
5. Evaluate the specificity of the affinity chromatography step.
© 2016 Sinauer Associates, Inc.
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Required Resources
Read/review the following resources for this activity:

Textbook: Chapters 7, 8
Lesson
Minimum of 2 scholarly source (in addition to the textbook)
Instructions
For this week’s assignment respond to one of the following options, and include Option 1, 2, or 3 as part of your heading.
Option 1: The first option is to name and describe in detail a key specific and recent healthcare technology. What are at least two key moral problems this technology creates? What are the proper moral guidelines for dealing with it in your view? Compare your approach to what an utilitarian and ethical egoist would say (each independently).
Option 2: In the second option, name and describe in detail a key specific and recent social technology. What are at least two key moral problems this technology creates? What are the proper moral guidelines for dealing with it in your view? Compare your moral approach to what an utilitarian and social contract ethicist would say (each independently).
Option 3: John Doe, Patient One, is in late stage of kidney disease. If he does not receive a new kidney, then he is predicted to die within a week. Doe is 45, single, and has no children. Doctors theorize that Doe damaged his kidney by not following a low-salt diet. Doe inherited one million dollars and is known for giving money to charity. Without a transplant, he will probably be forced to spend all his money searching for a kidney outside of the usual legal channels.  Patient Two is Jane Doe (no relation to John). Patient Two is a mother of two children (ages 21 and 24). She is divorced and 55 years old. She developed kidney problems due to eating a high-fat and high-sugar diet. If she does not receive a kidney within one month, doctors believe she will die.  Patient Three is an orphan. This orphan lives in a state facility. She was born with a genetic condition that constantly damages her kidney. The only known approach to her condition is to provide her with a kidney transplant every so often. She is 11 and has already undergone two kidney transplants. She will perish in two months if she does not receive another transplant.
All three patients are at the same hospital. The hospital only has one kidney to give out. The orphan’s birth parents were known to be of a religion that is opposed to organ donation. The other patients come from religions that do not oppose organ donation. Who should get the kidney?  Why should that candidate receive it over the others? Devise a course of social action and a solution for this case by using the ethics of egoism and then utilitarianism to a key moral conflict involving health care in this case. Appraise the interests of diverse populations (in terms  of ethnicity, race, religion, sexual orientation, etc.) and how they relate to the case.
For all the options:
Cite the textbook and incorporate outside sources, including citations.
You should not be using any text you used in a discussion board or assignment for this class or any previous class.
YOU WILL SUBMIT ALL OF THE FOLLOWING:
1. A written report that presents one of the options above (Be sure to give equal time to each element.)
2. An oral presentation that summarizes the report and examines the ethical ideas beyond the particulars of each case.
3. The link or a scan of the article mentioning the health technology, social technology, or case you are reporting on. If you made up the case, please indicate that in your report.
You should not be using any text you used in a discussion board or assignment for this class or any previous class.
Cite the textbook and incorporate outside sources, including citations.
Writing Requirements (APA format)
Length: 3-4 pages (not including title page or references page)
1-inch margins
Double spaced
12-point Times New Roman font
Title page
References page (minimum of 2 scholarly sources)
Presentation Requirements
Length: 2-3 minutes

Due 11/18/20
 
Review the “Reference List Example” in the topic study materials.
This assignment will help you prepare for the Topic 3 “Annotated Bibliography” and the Topic 4 “Three Pillars and Future Impact” assignments. Review the directions in Topic 3 and Topic 4 assignments for details.
Pick an industry (health care, marketing, retail, technology, etc.) with which you are familiar. Using Google Scholar, the GCU Library, or another reputable site, identify industry-relevant scholarly sources that support the three pillars concepts: servant leadership, ethics, and entrepreneurism. Refer to the topic study materials to determine what is considered a scholarly resource. Create a title page and a references list that cites nine references from the past five years. Include at the following types of references:

  • Five journal articles (include at least one article for each of the three pillars: servant leadership, ethics, and entrepreneurism)
  • One book
  • One website
  • One dissertation/thesis from a database
  • One book chapter

Prepare this assignment according to the guidelines found in the APA Style Guide, located in the Student Success Center. An abstract is not required.
Due 11/18/20
 
To complete this assignment, use “You Can Lead a Horse to Water but You Can’t Make Him Edit: Varied Effects of Feedback on Grammar across Upper-Division Business Students” and the guidelines for writing in the topic study materials.
Write a 250-500-word summary of the article that includes paraphrases of the article, the research problem, questions, method, findings, and implications discussed by the authors.
Prepare this assignment according to the guidelines found in the APA Style Guide, located in the Student Success Center. An abstract is not required.
Reminder: This is an academic exercise that should not be written in first person. This is an APA paper that includes a separate title page with running head and a reference page.
Due 11/25/20
 
Review the “Annotated Bibliography Example” and “Preparing Annotated Bibliographies” in the topic study materials.
This assignment will help you prepare for the Topic 4 “Three Pillars and Future Impact” assignment. Review the directions in Topic 4 assignment for details.
For this assignment, you will need three scholarly journal articles, one for each of the Three Pillars (servant leadership, ethics, and entrepreneurism) that may come from the “References List” assignment in Topic 2. In addition, locate scholarly journal articles that provide support for the Topic 4 assignment on the Three Pillars as they relate to the industry with which you are familiar. In your annotated bibliography, include the following:

  1. A brief description of the article.
  2. Main ideas from the article.
  3. How this source is relevant to your research paper.
  4. Why the article is considered scholarly.

While APA format is not required for the body of this assignment, solid academic writing is expected, and documentation of sources should be presented using APA formatting guidelines, which can be found in the APA Style Guide, located in the Student Success Center.
Due 12/2/20

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This paper will have an 8-page narrative (8 pages maximum – 5 point deduction for over page limit) plus a title page, abstract page, and reference list. 

You must use a minimum of four original research articles for your paper.

The purpose is to write a review of the literature on to answer your PICOT question using original research evidence. Discuss how this health problem is applicable in both the United States and a non-USA country, and appraise four original research studies on this topic.

This is a scholarly paper, written as if it was a “Review of the Literature” article published in a nursing journal. Select articles published within the past five years (2014 to present).
Do not use a non-peer review journal. Do not use a literature review article or systematic review article or clinical protocols as one of your articles. At least TWO of the four original research report articles MUST BE authored by nurses and published in a peer-reviewed nursing journal.  At least ONE of the two nursing research studies must address nursing practice in a non-USA country.

  • Page 1: Title page Create a title for your paper. Refer to your APA Manual.
  • Page 2: Abstract The abstract should open with a sentence that will serve as an introduction to and purpose of the paper. Then describe what is in each part of the paper. An abstract is usually a brief outline of the contents of the paper that typically does not exceed 250 words. At the end of the abstract place the phrase Key Words, and denote 3-4 keywords that would describe your paper (as for publication and indexing in CINAHL or PubMed).
  • Pages 3-11: Narrative
    • Heading: Significance and Background: Start the paper with a short paragraph that introduces the health problem in your PICOT question. (Approximately ½-page).
    • Heading: Purpose: Clearly define the global health problem, and discuss why it is important for nursing (why you are discussing this topic).  Discuss what is known about this problem in the United States and in a non-USA country.  For example, if the focus is adult hypertension, discuss guidelines for defining hypertension in the US and in a non-USA country.  (Approximately 1 page).
    • Heading: Methods: Discuss the methods used to select the following research studies.
      Next use the subheading:
      • Sub-headings: Study #1: Nursing research Study in the USA (Approximately 1½ pages)
      • Sub-headings: Study #2: Nursing Research Study Non-USA (Approximately 1½ pages)
      • Sub-headings: Study #3: Nursing Research Study USA or Non-USA (Approximately 1½ pages)
      • Sub-headings: Study #4: Nursing Research Study USA or Non-USA (Approximately 1½ pages)
    • Heading: Conclusion.  Summarize the key points from your discussion. How would you translate (use) the new evidence found from your appraisals to change practice or answer your PICOT question? (Approximately 1 page).
  • Page 12+: Reference page: Refer to your APA Manual.

This review is a scholarly paper in APA format and with at least three APA references (two articles and textbook), cited both in the text and the reference page.

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  • PostoperativePainManagementClinicalPracticeGuidelines.pdf
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WEEK 4 – DISCUSSION

What do you believe to be the most compelling reason to migrate information to the cloud? What is your biggest security concern about doing so?
It should be in between 250-300 words

RESEARCH ASSIGNMENT – WK 4

Compare and contrast two difference cloud computing services (Amazon Web Service and Microsoft Azure). Explain the differences and the similarities and select your choice of providers if you had to make the decision for your business. Write up a comparison on the services offered (2 pages maximum). Once again, it is essential that you use your own words (do not just copy and paste from the Internet).
These two links may offer some additional information for this assignment but you are encouraged to use additional sources for your project/assignment.
https://aws.amazon.com/security/introduction-to-cloud-security/
https://azure.microsoft.com/en-us/

WEEK 4 ASSIGNMENT

  • 4.1 List ways in which secret keys can be distributed to two communicating parties.
  • 4.2 What is the difference between a session key and a master key?
  • 4.3 What is a key distribution center?
  • 4.4 What entities constitute a full-service Kerberos environment?
  • 4.5 In the context of Kerberos, what is a realm?
  • 4.6 What are the principal differences between version 4 and version 5 of Kerberos?
  • 4.7 What is a nonce?
  • 4.8 What are two different uses of public-key cryptography related to key distribution?
  • 4.9 What are the essential ingredients of a public-key directory?
  • 4.10 What is a public-key certificate?
  • 4.11 What are the requirements for the use of a public-key certificate scheme?
  • 4.12 What is the purpose of the X.509 standard?
  • 4.13 What is a chain of certificates?
  • 4.14 How is an X.509 certificate revoked?

Complete your answers on a WORD Document,

This assignment will incorporate a common practical tool in helping clinicians begin to ethically analyze a case. Organizing the data in this way will help you apply the four principles of principlism.

Based on the “Case Study: Healing and Autonomy” and other required topic study materials, you will complete the “Applying the Four Principles: Case Study” document that includes the following:

Part 1: Chart

This chart will formalize principlism and the four-boxes approach by organizing the data from the case study according to the relevant principles of biomedical ethics: autonomy, beneficence, nonmaleficence, and justice.

Part 2: Evaluation

This part includes questions, to be answered in a total of 500 words, that describe how principalism would be applied according to the Christian worldview.

Remember to support your responses with the topic study materials.

APA style is not required, but solid academic writing is expected.

You are required to submit this assignment to LopesWrite. Refer to the LopesWrite Technical Support articles for assistance.

PHI-413V-RS-T3ApplyingFourPrinciplesCaseStudy.docx

Please answer the following Discussion it is due on Tomorrow night. Thanks so much. My time here is 4:00 pm. 

Assignment 6: Fantasy Campaign

  • Evaluate your marketing needs against your marketing budget. (CO5)
  • Generate a marketing and advertising strategic plan to meets the goals of your venture. (CO7)

For Assignments 4 and 5, you worked on a marketing campaign based on a budget that you calculated. For this assignment, design a marketing campaign based on a marketing campaign budget of $100,000 for your product/service. Yes, you get to design the campaign of your dreams. This assignment opens your eyes to various marketing opportunities using a larger budget. From this assignment, new ideas can form that you could gradually implement later on.
For this assignment:

  1. The objective should include (but not limited to):
    1. Title of the campaign. (What is your slogan?)
    2. What do you want the campaign to achieve? Be detailed, this is not as simple as I want more customers. What type of customers? Number of products/services to be sold?
    3. Be sure to state the positioning statement.
  2. Propose how you will measure success? (What metrics will you use?)
  3. Design the campaign. (Provide detail.)
    1. What is the advertising type (broadcast, outdoor, print, public service, product placement, and online/mobile)? There may be more than one focus.
    2. What is the advertising scenario? (Remember the slogan created in Assignment 4.)
  4. Explain why you selected this type of campaign (advertising type(s) and scenario)? (Why is this campaign appropriate for customer segment?) Provide detail.
  5. Determine how the campaign budget will be spent.
  6. The assignment should be 3-4 pages (excluding the title, abstract, and reference pages) and follow APA formatting.
  7. Support your statements with at least two peer-reviewed resources (excluding textbooks). Cite your sources in APA format.
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Question Description

Theory–Practice Gap

Objectives:

Fundamentals of Nursing Models, Theories, and Practice discusses the theory-practice gap in detail in many chapters. As you’ve read throughout the course, there is ongoing discussion about the connection between theory and practice, and the application in day-to-day nursing activities. This assignment is designed to illustrate that although there may be a gap, other factors play an important role in decision-making and each aspect of theory, research, and practice experience are integral to well-rounded patient care. review Figure 1.4 Correlation: education, science and practice.

Think of a scenario in which theory, research, and practice interact to create good patient outcomes.

Create a visual representation of the theory-practice relationship or gap by replacing the text in the Theory-Practice Gap diagram template:

Note: Use names, citations, or brief explanations only. The narrative summary should include descriptions and more detailed discussion about each concept in the scenario.

Write a minimum of 525- to 700-word narrative explanation of your visual representation following the diagram.

• Describe the chosen theory, research, and practice guideline or standard.

• Explain the relationship between the three and discuss the role each plays in quality patient care in the scenario.

• Explain any gaps, such as a lack of research, no practice standard, or no useable theory.

• Determine the best course of action for making decisions in the absence of one aspect.

Include documentation of the practice guideline or standard, and your corresponding research, evidence, or literature example.

Cite your research and practice guidelines in-text and on the references page at the end of the template.

I have included the template in the wk 5 assignment file I uploaded